Post on 07-Jul-2018
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METABOLISME PROTEIN ASAMAMINO
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ASAM AMINOPROTEINkimia
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masalah
Prinsip protein –
1 struktur dasar AA yang menyusun protein – 2 bagaimana di diserab dalam bentuk apa
– 3 Bagaimana pembentukan dalam sel (tubuh) ?
– 4 Apa sisa metabolismenya
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H2NAminogroup C
Side hainR
H
C
O
OH
Car!o"#lgroup
Non$ioni%ed &orm
STRUCTUR ASAM AMINO
Radikal = !"3# komple$#
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Amino Aids
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20 Amino
Acids
Note:
Variable
R Group
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C
C
OH
O
HN
H2N
O
HO
H
H
H
CH'
C$(erminus
N$(erminus
glisin
Alanin
C
C
Iatan peptida
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H2N
H
H
C C
O
OHCar!o"#lgroup
H2N
H
CH'
C C
O
OHAminogroup
H2N
H
H
C
O
C N CC
HH
CH'OH
O
Pep(ide!ond
) H2O)
!ORMATION O! A "I#$#TI"$ %& 'A& O! A #$#TI"$ %ON"
alaninglisin
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HH H H H H HO O O H H O H H ON C C N NC C C C
H CH' CH2
OH
N$(erminus
N C C
CH2
C
O
OH
CH2
N C C
CH
CH'H'C
CH2
OH
H H ON C C
H H ON C C
H H ON C C
CH2
SH
OH
C$(erminus
#O($#TI"$ C)AIN
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H
H H H H H HO O O H H O H H O
N C C N NC C C C
H CH' CH2
OH
N$(erminus
N C C
CH2
C
O
OH
CH2
N C C
CH
CH'H'C
CH2
OH
H H O
N C C
H H O
N C C
H H O
N C C
CH2
SH
OH
C$(erminus
H2N
H
H
C C
O
OHCar!o"#lgroup
H2N
H
CH'
C C
O
OHAminogroup
H2N
H
H
C
O
C N CC
HH
CH'OH
O
Pep(ide!ond
) H2O
H2N *l# Ala Ser Asp
N$(erminus
2+ ' ,- . / 0
Phe 1al T#r C#s
C$(erminus
COOH
)
SUMMAR& O! polipeptida STRUCTUR$
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Protein makanan% A&tin dan miosin (otot) asam amino% Asam amino darah sel
– 'ergantung kebutuhan# BR
omposisi makanaMakanan
En%im penernaan
Pep(on dan pep(idaseembentuk protein
Energi
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TABLE '32 A Summar# o& Pro(ein S(ru(ure
Le4el 5esrip(ion
Primar#
Seondar#
Ter(iar#
The se6uene o&
amino aids
7orma(ion o&
$helies and
$plea(ed shee(s
O4erall (hree$
dimensional shape
o& a pol#pep(ide
8ua(ernar# Shape produed !#
om!ina(ions o&
pol#pep(ides
S(a!ili%ed !#9
Pep(ide !onds
H#drogen !onding !e(:een
pep(ide groups along (he
pep(ide !ak!one
Bonds and o(her in(era(ions
!e(:een R$groups; or
!e(:een R$groups and (he
pep(ide !ak!one
Bonds and o(her in(era(ions
!e(:een R$groups; and
!e(:een pep(ide !ak!ones
o& di&&eren( pol#pep(ides
*l# Ser Asp *ls
E"ample9 Hemoglo!in
#ROT$IN STRUCTUR$
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Protein STRUCTUREGoegle ;you tube
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STRUCTURE Protein*lo!ular and 7i!rous Pro(eins
7i!rous Pro(eins –
ittle or no tertiary stru&ture% – ong parallel polypeptide &hains%
– !ross linkages at inter*als +orming long +ibres or
sheets%
–
,sually insoluble% – any ha*e stru&tural roles%
– -%g% keratin in hair and the outer layer o+ skin#
&ollagen (a &onne&ti*e tissue)%
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STRUCTURE Protein*lo!ular and 7i!rous Pro(eins
*lo!ular Pro(eins –
"a*e &omple$ tertiary and sometimes .uaternarystru&tures%
– /olded into spheri&al (globular) shapes%
– ,sually soluble as hydrophobi& side &hains in
&entre o+ stru&ture% – Roles in metaboli& rea&tions%
– -%g% en0ymes# haemoglobin in blood
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AMINO ACI5 BIOS?SE O7 COMMON INTERME5IATES@
,!- ,!P"P"A'- R5B6P"7 "5
↓
↓
3P"P"8!-RA'- -R59-
↓ ↓
↓ 8!59-
-4P" : P-P !8'-59-
↓ ↓
P"-7'8R P8R,;A'-
AA 'RP ↓ ;A
!5'RA'- -,# 5-
<
AA!-'A'-# α>-','ARA'-
AP# A9# ,# 9# PR# AR# 8# '"R# -'
Ana!olisme
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!opyright 2@ ohn iley Cons# 5n&%
Pro(ein S#n(hesis
1@
Gene expression: information coded in a gene is
used to synthesize a product—a protein or a
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Protein dalam tubuh
A&tin dan miosin otot
Albumin
sel dan darah lobulin anitbodi dalam darah
"b transport oksigen
ioglobulin transport oksigen di otot
!ollagen Daringan tendo# ligament dll
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En%im
heksokinase
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Mioglo!in
o(o(
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Makanan(protein)
Asam Amino
HATI
ASAM ETO
?REA
% A 5'RA'SEL T?B?H
--95A
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Alanine &arries amino groups +rom mus&le to the
li*er +or e$&retion
Ala
$ke(o$
glu(ara(e
p#ru4a(e
CO2$
CO
CH'
*lu
NH-)
gluose
CO2$)H'N C H
CH'
urea
li4er
Ala $ke(o$
glu(ara(e
muslepro(ein
p#ru4a(e
CO2$
CO
CH'
*lu
amino aids
gluose
CO2$)H'N C H
CH'
musle !lood O
H2N$C$NH2
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'he amino groups o+ glutami& a&id and glutamine
&an be released as ammonia in li*er mito&hondria
H2ONA5) or
NA5P)
*lu
$ke(o
aids
ellular
pro(ein
inges(ed
pro(ein
NA5H or NA5PH ) H)
$ke(o$glu(ara(e
CO2$
)H'N C H
R
CO2$
CO2$
COCH2
CH2
CO2$
CO2$
)
H'N C HCH2CH2
CO2$
CO
Ramino
aids
*ln &rom
musle
o(her (issue
NH-) Bu( ammonia is (o"i;
par(iularl# (o neural (issue3
Organisms mus( ge( rid o& i(3
CONH2
CO2$
)H'N C H
CH2
CH2
(ransaminases
glu(ama(e
deh#drogenase
NH-)
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AMINO ACI5 5E*RA5ATION INTERME5IATES
CO2
CO2
Pyru*ate
A&etyl!oA A&etoa&etate
!itrate
5so&itrate
ketoglutarateu&&inyl!oA
/umarate
$aloa&etate
!itri&
A&id!y&le
CO2
lu&ose
Ala er
!ys 'hrEly 'rpE
5leE
euF
ysF
'hrE
euF 'rpE
ysF 'yrE
PheE
Asn
Asp
Asp
PheE'yrE
5leE
et
;al
Arg "is
lu Pro
ln
*luogeni
e(ogeni
Both lu&ogeni& and >etogeni&F Purely >etogeni&
http://var/www/apps/conversion/tmp/scratch_3/glycolysis.ppthttp://var/www/apps/conversion/tmp/scratch_3/glycolysis.ppt