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Fatma AlqudsiAnimal cell and tissue culture Bio357

Small tissue culture laboratory

Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.

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Fatma Alqudsi Animal cell and tissue cultureBio 357

Medium sized tissue culture

laboratory

Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.

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Fatma Alqudsi Animal cell and tissue cultureBio 357

Tissue culture laboratory with adjacent room

Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.

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Fatma Alqudsi Animal cell and tissue cultureBio 357

Large tissue culture laboratory

Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.

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Fatma Alqudsi Animal cell and tissue cultureBio 357

Hot Room

Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.

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Fatma Alqudsi Animal cell and tissue cultureBio 357

Washing upsink

Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.

8/7/2019 ACC Lab Setup

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Fatma Alqudsi Animal cell and tissue cultureBio 357

Liquid nitrogen

store

Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.

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Fatma AlqudsiAnimal cell and tissue culture Bio357

Minimum requirements Desirable features Useful additions

Sterile area, clean, quiet, and with no

through traffic

Filtered air (air-

conditioning)Piped CO2 and compressed air

Separate from animal house and

microbiological labs

Service bench adjacent to

culture areaStoreroom for bulk plastics

Preparation area Separate prep room Quarantine room

Wash up area (not necessarily within

tissue culture laboratory, but at leastadjacent to it)

Hot room with temperature

recorder

Containment room (could

double as quarantine room)

Space for incubator(s) Separate sterilizing room

Liquid N2 storage tank (~500 L)

and separate storeroom for

nitrogen freezers

Storage areas: Separate cylinder store Microscope room

Liquids: ambient, 4C, 20C Darkroom

Glassware (shelving) Vacuum line

Plastics (shelving)

Small items (drawers)

Specialized equipment (slow turnover),

cupboard(s)

Chemicals: ambient, 4C, 20C; share

with liquids, but keep chemicals in

sealed container over desiccant

CO2 cylinders

Space for liquid N2 freezer(s)

Sink

Tissue culture facilities

Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.

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Fatma Alqudsi Animal cell and tissue cultureBio 357

Equipment of tissue

culture Laboratory

Equipment of tissueEquipment of tissue

culture Laboratoryculture Laboratory

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Fatma Alqudsi Animal cell and tissue cultureBio 357

Requirements of a tissue

culture laboratory

1. Laminar-flow hood

2. Sterilizing oven3. Autoclave4. Centrifuge

5. Balance6. Cell counter7. Inverted microscope8. Dissecting microscope

9.Humid Co2 incubator10.Incubator11.pH meter12.Hotplate magnetic

stirrer13.Magnetic stirrer14.Glassware15.Refrigerator

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Fatma Alqudsi Animal cell and tissue cultureBio 357

http://www.pemed.com/lab/hoods/forma110401_47.jpg

Laminar-flow hood

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Fatma Alqudsi Animal cell and tissue cultureBio 357

http://www.geol.ku.dk/pershps/robertfrei/WEB/laminair-flow-hood-1.jpg

Laminar-flow hood

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Fatma Alqudsi Animal cell and tissue cultureBio 357

Inverted microscope

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Fatma Alqudsi Animal cell and tissue cultureBio 357

http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artjul98/invert.html

Inverted microscope

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Fatma Alqudsi Animal cell and tissue cultureBio 357

Inverted microscopehttp://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artjul98/invert.html

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A device in which solid or liquid

particles of different densities are

separated by rotating them in a

tube in a horizontal circle. The

denser particles tend to move

along the length of the tube to agreater radius of rotation,

displacing the lighter particles to

the other end.

html.centrifuge/C/.../info.daviddarling.www

Centrifuge

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Fatma Alqudsi Animal cell and tissue cultureBio 357

https://reader009.{domain}/reader009/html5/0523/5b04c15f85524

Centrifuge