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Frédér ic Silvestre at a glance… Systems Ecotoxicology Laboratory University of Namur FUNDP - URBE Rue de Bruxelles 61 5000 Namur Belgium frederic.si lvestre@fundp.ac.be

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Licenses & Thesis

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Annexes Frédéric Silvestre

Thesis and main cursus

Ph.D thesis in Biological Sciences (University of Namur FUNDP; 22 of march 2005) « Stress response to cadmium in an euryhaline crustacean, Eriocheir sinensis. An integrative approach including analysis of the proteome ». http://edoc.bib.ucl.ac.be:61/ETD-db/collection/available/FUNDPetd-06222005-152107/ Jury : Pr. P. Devos (FUNDP - Promotor) ; Pr. M. Raes (FUNDP - President) ; Pr. R. Blust

(University of Antwerp) ; Pr. P. Kestemont (FUNDP) ; Dr. A. Péqueux (University of Liège).

Master degree in Environmental Management (Free University of Brussels ULB - IGEAT ; 1998) + “eco-adviser” « Ecotourism in Antarctica : a tool for sustainable development ? »

High Distinction Promotors: Dr. M.-F. Godart - ULB and Dr. C. DeBroyer – Royal Institute of Natural Sciences IRSNB.

“Licence” in Zoological Sciences (University of Liège - ULg ; 1996) « Heavy metals in Sarpa salpa (L. 1758). Relations with feeding. » The Highest Distinction Promotor: Pr. J.-M. Bouquegneau.

Bachelor degree in Biological Sciences (University of Liège – ULg ; 1994)

High school diploma at the Royal Athenee “Air Pur” in Seraing (B) (1992)

Other trainings and certificates

! From Research to Business – 6 days training for technology start-up company

management – Louvain School of Management; HEC Liège; Solvay Brussels Business School – March 2010

! Good Laboratory Practices – Training BioWin, The Health Cluster of Wallonia – Cefochim – Seneffe, Belgium – 7 and 8th of May 2009

! Green Technology Entrepreneurship Academy – one week training for green technology start-up company management - University of California Davis School of Entrepreneurship (USA) ; July 7 – 11, 2008

! Training at the University of Odense (Denmark) : « Advanced methods in protein chemistry and mass spectrometry ». Pr. Ole N. Jensen, Protein Research Group ; November 5 – 18, 2001

! Certificate in accounting – University of Liège – 1997 ! Certificate in statistical analysis – University of Liège – 1996

Employers

! University of Namur FUNDP (B) : Assistant Professor – 2010 to undetermined ! FNRS (Fonds National de la Recherche Scientifique – B) – Post-doc researcher (chargé de

recherche) – 1/2007 to 09/2010 ! University of Namur FUNDP (B) :

• Maître de Conférence – 2007 to 2010 (annually renewed) • Senior Researcher – 9/2006 to 12/2006 • Researcher on a BSPO (Belgian Scientific Policy) project – 9/2004 to 8/2006 • Teaching assistant – 9/1998 to 8/2004

International background

Institutional mission for the University of Namur in Benin (University of Cotonou and Parakou). Research projects in biology and sustainable development setup. (10 days in 2012). Post-doctoral researcher at the University of California Davis (UC Davis – USA) – Pr. D. Kültz – Department of Animal Science. "Analysis of stress proteomes in aquatic species exposed to environmental stressors". (6 weeks in 2010). Post-doctoral researcher at the University of Louisiana Lafayette (ULL – USA) – Pr. P. Klerks – Department of Biology. "Mechanisms of acclimation to cadmium in the least killifish Heterandria formosa". (2 months in 2009). Post-doctoral researcher at the University of California Davis (UC Davis – USA) – Pr. D. Kültz – Department of Animal Science. "Analysis of stress proteomes in aquatic species exposed to environmental stressors". (7 months in 2008). Post-doctoral researcher at the University of Louisiana Lafayette (ULL – USA) – Pr. P. Klerks – Department of Biology. "Mechanisms of acclimation to cadmium in the least killifish Heterandria formosa". (3 months 2007). Scientific mission in Vietnam within a scientific collaboration project between Vietnam and Belgium (1 month in 2006) : international meeting in Ho Chi Minh City ; teaching in CanTho University and Hanoi University; research work and improvement of a biochemistry laboratory in CanTho University ; sampling. Scientific mission in Vietnam within a scientific collaboration project between Vietnam and Belgium (1 month in 2005) : research work in CanTho University ; sampling.

Scientific awards and grants

Fulbright Advanced Research grant for a post-doctoral program in UC Davis (2008). Travel grants from FNRS (Fonds de la Recherche Scientifique) for post-doctoral programs in UL Lafayette and UC Davis (2007, 2008, 2009 and 2010). Co-author of the « Best Poster Award » at the BELTOX annual meeting, Louvain-La-Neuve (Belgium), November 2010. “Looking for stable biomarkers in the PBMC of Asian catfish, Pangasianodon hypophthalmus, following malachite green exposure using a proteomic approach ”. M.-A. Pierrard, P. Kestemont, E. Delaive, M. Dieu, N.T. Phuong, M. Raes and F. Silvestre. Co-author of the « Best Poster Award » at the BELTOX annual meeting, Woluwé (Belgium), 28th November 2008. “Chronic exposure to aroclor 1254 impairs expression profiles in the developing brain of xenopus laevis tadpoles”. Gillardin, V., Silvestre, F., Roland, K., Raes, M., Kestemont, P. Co-author of the « Young Scientist Award » poster at the 16th SETAC Europe annual meeting Europe, Den Haag (The Netherlands), 7-11 may 2006 : « Effects of the PCBs mixture Aroclor® 1254 on antioxidant systems and protein expression profiles of developing Xenopus laevis tadpoles ». Gillardin V., Divoy C., Silvestre F., Forget MC, Kestemont, P. Travel grant "Fonds Adrien Bauchau" – FUNDP 2004.

International publications with peer review (35) (Scopus h index 10 ; citations 295 ;

average number of citations 8.4 ; cum IF: 92.8 ; avg IF: 2.65) Under revision or submitted (by September 2012) : A controversial triarylmethane dye, the malachite green: Review. M-A Pierrard, P Kestemont, F Silvestre. Ecotoxicology. Submitted. IF: 3.051. Effects of Roundup® on the protein expression patterns in the liver of the metamorphosing common frog Rana temporaria. V. Gillardin, A. Oriol, M. Denoël, E. Delaive, M. Dieu, M. Raes, E. DePauw, F. Silvestre, P. Kestemont. Aquat. Toxicol. Submitted. IF: 3.761. Biomarkers of physiological status in the Asian catfish, Pangasianodon hypophthalmus, following malachite green treatment. M-A. Pierrard, P. Kestemont, W. Sanchez, N.T. Phuong, F. Silvestre., Aquaculture. Submitted. IF: 2.044. Heat acclimation of European bullhead interacts with subsequent cadmium exposure resulting in a specific proteome response. Dorts, J., Kestemont, P., Marchand, P.-A., D’Hollander, W., Thézenas, M.-L., Raes, M., Silvestre, F., J. Proteomics. Submitted. IF: 4.878.

Published or in press as first or last author : Proteomics to assess the role of phenotypic plasticity in aquatic organisms exposed to pollution and global warming. F. Silvestre, V. Gillardin, J. Dorts. Int. Comp. Biology. In press. IF: 2.447. Fish peripheral blood mononuclear cell preparation for future monitoring applications. M-A. Pierrard, K. Roland, P. Kestemont, F. Silvestre. Anal. Biochem. Meth. Biol. Sci. 426 (2012), 153-165. IF: 2.996. Proteomic analysis of blood cells in fish exposed to chemotherapeutics: Evidence for long term effects. Pierrard, M-A., Kestemont, P., Phuong, N.T., Delaive, E., Thezenas, M-L., Dieu, M., Raes, M., Silvestre, F., J. Proteomics. 75 (2012), 2454-2467. IF: 4.878. Malachite green toxicity assessed on Asian catfish primary cultures of peripheral blood mononuclear cells by a proteomic analysis. M-A. Pierrard, P. Kestemont, E. Delaive, M. Dieu, M. Raes, F. Silvestre, Aquat. Toxicol. 114-115 (2012), 142-152. IF: 3.761. Proteasome and antioxidant responses in Cottus gobio during a combined exposure to heat stress and cadmium. Dorts, J., Bauwin, A., Kestemont, P., Jolly, S., Sanchez, W., Silvestre, F. Comp. Biochem. Physiol. C. 155 (2012), 318-324. IF: 2.616. Ecotoxicoproteomics in gills of the sentinel fish species, Cottus gobio, exposed to perfluorooctane sulfonate (PFOS). Dorts, J., Kestemont, P., Marchand, P.-A., D’Hollander, W., Thézenas, M.-L., Raes, M., Silvestre, F. Aquat. Toxicol. 103 (2011), 1-8. IF: 3.761. Proteomic response to sublethal cadmium exposure in a sentinel fish species, Cottus gobio. Dorts, J., Kestemont, P., Dieu, M., Raes, M., Silvestre, F. J. Proteome Res., 10 (2011), 470-478. IF: 4.878. A differential proteomic approach to assess the effects of chemotherapeutics and production management strategy on giant tiger shrimp Penaeus monodon. Silvestre Frédéric, Tu Huynh Thi, Bernard Amandine, Dorts Jennifer, Dieu Marc, Raes Martine, Phuong Nguyen Thanh , Kestemont Patrick. Comp. Biochem. Physiol. D., 5 (2010), 227-233. IF: 1.758. A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium. Frédéric Silvestre, Javier Linares-Casenavea, Serge I. Doroshov, Dietmar Kültz. Sci. Tot. Environ., 408 (2010), 3176-3188. IF: 2.579. Is HSP70 involved in acclimation to cadmium in the Chinese mitten crab, Eriocheir sinensis ? F. Silvestre, G. Trausch, P. Devos. Bull. Environ. Contam. Toxicol., 78 (2007), 432-435. IF : 0.626. Differential protein expression profiles in anterior gills of Eriocheir sinensis during acclimation to cadmium.

F. Silvestre, J.-F. Dierick, V. Dumont, M. Dieu, M. Raes, P. Devos. Aquat. Toxicol., 76 (2006), 46-58. IF : 2.964. Tissue-specific cadmium accumulation and metallothionein-like protein levels during acclimation process in the Chinese crab Eriocheir sinensis. F. Silvestre, C. Duchêne, G. Trausch, P. Devos. Comp. Biochem. Physiol. C, 140 (2005), 39-45. IF: 1.456. Hyper-osmoregulatory capacity of the Chinese mitten crab (Eriocheir sinensis) exposed to cadmium. Acclimation during chronic exposure. F. Silvestre, G. Trausch, P. Devos. Comp. Biochem. Physiol. C, 140 (2005), 29-37. IF: 1.456. Uptake of Cadmium through isolated perfused gills of the Chinese mitten crab, Eriocheir sinensis. F. Silvestre, G. Trausch, A. Péqueux, P. Devos. Comp. Biochem. Physiol. A, 137 (2004), 189-196. IF: 1.635. Effects of atrazine on osmoregulation in the Chinese mitten crab, Eriocheir sinensis. F. Silvestre, G. Trausch, L. Spàno, P. Devos. Comp. Biochem. Physiol. C, 132 (2002), 385-390. IF: 0.979. Published or in press as second author : Combined effects of deltamethrin, temperature and salinity on oxidative stress biomarkers and acetylcholinesterase activity in the black tiger shrimp (Penaeus monodon). Tu, H. T., Silvestre, F., Phuong, N.T., Kestemont, Chemosphere. 86 (2012), 83-91. IF: 3.206. A multi-biomarker approach to assess the impact of farming systems on black tiger shrimp (Penaeus monodon). Tu, H. T., Silvestre, F., Bernard A., Douny, C., Phuong, N.T., Tao, C.T., Maghuin-Rogister, G., Kestemont, P. Chemosphere, 81 (2010), 1204-1211. IF: 3.155. Differential protein expression profile in the liver of pikeperch (Sander lucioperca) larvae fed with different phospholipid levels. Hamza Neïla, Silvestre Frédéric, Mohamed Mhetli, Ines Ben Khemis, Marc Dieu, Kestemont Patrick. Comp. Biochem. Physiol. D., 5 (2010), 130-137. IF: 1.758. Effects of pesticides and antibiotics on penaeid shrimp with special emphases on behavioural and biomarker responses. H.T. Tu, F. Silvestre, N. T. Phuong, P. Kestemont. Environ. Toxicol. Chem., 29 (2010), 929-938. IF: 2.420. Acetylcholinesterase activity as a biomarker of exposure to antibiotics and pesticides in the black tiger shrimp (Penaeus monodon). Tu, H. T., Silvestre, F., Scippo, M.-L., Thomé, J.-P., Phuong, N.T., Kestemont, P. Ecotox. Environ. Saf., 72 (2009), 1463-1470. IF: 2.014.

Protein expression profiling in the African clawed frog Xenopus laevis tadpoles exposed to the PolyChlorinated Biphenyls mixture Aroclor 1254. V. Gillardin, F. Silvestre, M. Dieu, E. Delaive, M. Raes, J.-P. Thomé, P. Kestemont. Mol. Cell. Proteomics, 8 (2009), 596-611. IF: 8.791. Oxidative stress response of black tiger shrimp (Penaeus monodon) to enrofloxacin and to culture system. Tu, H. T., Silvestre, F., Bernard A., Douny, C., Phuong, N.T., Tao, C.T., Maghuin-Rogister, G., Kestemont, P. Aquaculture, 285 (2008), 244-248. IF: 1.735. Effects of Aroclor 1254 on oxidative stress in developing Xenopus laevis tadpoles. V. Gillardin, F. Silvestre, C. Divoy, J.-P. Thomé, P. Kestemont. Ecotox. Environ. Saf., 72 (2008), 546-551. IF : 2.014. Oxidative stress, protein carbonylation and heat shock proteins in the giant tiger shrimp Penaeus monodon exposed to endosulfan or deltamethrin. Dorts, J., Silvestre, F., Tyberghein, A.-E, Tu, H.T., Douny, C., Maghuin-Rogister, G., Phuong, N., Kestemont, P. Environ. Toxicol. Pharmacol., 28 (2009), 302-310. IF: 1.051. Published or in press as co-author : Physiological and proteomic responses to single and repeated hypoxia in juvenile Eurasian perch under domestication - Clues to physiological acclimation and humoral immune modulations. Douxfils, J., Mandiki R., Milla S., Mathieu C., Henrotte E., Silvestre F., Rougeot C., Raes M., Kestemont P. Fish Shellfish Immunol. In press. IF: 3.322. Combined analysis of Perca fluviatilis reproductive performance and oocyte proteomic profile. M.-D. Castets, B. Schaerlinger, F. Silvestre, J.-N. Gardeur, C. Corbier, P. Kestemont, P. Fontaine., Theriogenology. 78 (2012), 432-442. IF: 2.045. Evidence that elevated water temperature affects the reproductive physiology of the European bullhead Cottus gobio. Dorts, J., Grenouillet, G., Douxfils, J., Mandiki, R., Milla, S., Silvestre, F., Kestemont, P.,. Fish Physiol. Biochem. 38 (2012), 389-399. IF: 1.232. Physiological and proteomic evidences that domestication process differentially modulates the immune status of juvenile Eurasian perch (Perca fluviatilis) under chronic confinement stress. Douxfils, J., Mathieu, C., Mandiki, S.N.M., Milla, S., Henrotte, E., Wang, N., Vandecan M., Dieu M., Dauchot N., Pigneur L-M., Li X., Rougeot, C., Mélard, C., Silvestre, F., Van Doninck K., Raes M., and Kestemont, P., Fish Shellfish Immunol. 31 (2011), 1113-1121. IF: 3.044. Does domestication process affect stress response in juvenile Eurasian perch Perca fluviatilis?

Douxfils J, Mandiki SN, Marotte G, Wang N, Silvestre F, Milla S, Henrotte E, Vandecan M, Rougeot C, Mélard C, Kestemont P. Comp. Biochem. Physiol. A. 159 (2011), 92-99. IF: 2.235. Eco-, geno- and human toxicology of bio-active nanoparticles for biomedical applications. Johan Robbens, Caroline Vanparys; Ingrid Nobels; Ronny Blust; Karen Van Hoecke; Colin Janssen; Karel De Schamphelaere; Kathleen Roland; Gersande Blanchard; Frédéric Silvestre; Virginie Gillardin; Patrick Kestemont; Roel Anthonissen; Olivier Toussaint; Christelle Saout; Sébastien Vankoningsloo; Ernesto Alfaro-Moreno; Peter Hoet; Laetitia Gonzalez; Peter Dubruel; Paul Troisfontaines. Ecotoxicology, 269 (2010), 170-181. IF: 2.836. Adverse effects of enrofloxacin when associated with environmental stress in Tra catfish (Pangasianodon hypophthalmus). Wang Neil, Nkejabega Noemie, Hien Nguyen-Ngoc, Silvestre Frederic, Huynh Thi-Tu, Phuong Nguyen-Thanh, Kestemont Patrick, Huong Do-Thi-Thanh. Chemosphere, 77 (2009), 1577-1584. IF: 3.253. Combined effects of herbicides on biomarkers reflecting immune-endocrine interactions in goldfish. Part I: Immune and antioxidant effects. Fatima, M., Mandiki, S.N.M., Douxfils, J., Silvestre, F., Coppe, P., Kestemont, P. Aquat. Toxicol. 81 (2007), 159-167. IF: 2.964. Do Sewage Treatment Plant Discharges Substantially Impair Fish Reproduction in Polluted Rivers? Douxfils Jessica, Mandiki Robert, Silvestre Frederic, Bertrand Arnaud, Leroy Delphine, Thomé Jean-Pierre, Kestemont Patrick. Sci. Total Environ., 372 (2007), 497-514. IF: 2.224. Multiscale approach of fish responses to different types of environmental contaminations: a case study. Mayon N, Bertrand A, Leroy D, Malbrouck C, Mandiki SNM, Silvestre F, Goffart A, Thomé JP, Kestemont P. Sci. Total Environ., 367 (2006), 715-731. IF: 2.359. Effects of atrazine on sex steroïd dynamics, plasma vitellogenin concentration and gonad development in adult Goldfish (Carassius auratus). L. Spanò, S.N.M. Mandiki, R. van Aerle, C.R. Tyler, F. Silvestre, P. Devos, J.-P. Thomé, P. Kestemont. Aquat. Toxicol., 66 (2004), 369-379. IF: 2.418. Other publications

Investigation on the use of drugs and chemicals in shrimp farming and the kinetics of Enrofloxacin and Furazolidone in black tiger shrimp (Penaeus monodon). Tu, H. T., Phuong, N.T., F, Silvestre, C. Douny, Tao, C.T., G. Maghuin-Rogister, P. Kestemont. Scientific Journal, Cantho University (2006) (In Vietnamese).

Research Reports

« Mécanismes d'acclimatation et d'adaptation au cadmium chez des organismes aquatiques. Analyse sub-protéomique du réticulum endoplasmique et héritabilité des phénotypes observés. Implication de mécanismes épigénétiques ». Rapports annuels d’activité au FNRS (2006-2007, 2007-2008, 2008-2009).

« Improvement of shrimp production sustainability and safety in Vietnam ». A joint vietnamese-belgian project funded by BELSPO. Final report (April 2004- April 2006). « Improvement of shrimp production sustainability and safety in Vietnam ». A joint vietnamese-belgian project funded by BELSPO. First annual report (April 2004-April 2005).

Conferences

1° Organizer Organizer of the first conference “Ecotoxicology – Wallonia”, on October 25th in Namur, Belgium. It aimed at confronting the research topics of main teams involved in ecotoxicology in the Walloon Region, Belgium. Prof. George Somero, from Stanford University, was invited and gave a conference entitled “Using mussel power to study ecological and evolutionary physiology”. http://webapps.fundp.ac.be/system-ecotox/?page_id=23

2° Invited speaker “Mechanisms of acclimation to pollutants and elevated temperature in aquatic organisms. Changes of the cellular phenotype revealed by proteomics.” Silvestre F. SICB (Society for Integrative and Comparative Biology) Annual Meeting 2012 in Charleston, SC. “Proteome changes during acclimation of aquatic organisms to environmental stress.” Silvestre F., Dorts J., Dubois A. First International Conference on Environmental OMICS in Guangzhou, China, 8th-12th November 2011. “Mechanisms of action of toxicants on aquatic animals. Does expression proteomics reveal general stress response or specific mechanisms ?” Silvestre F. PROTEOMLUX meeting, Luxembourg, November 2011. “Mechanisms of action of toxicants on aquatic animals. Does expression proteomics reveal general stress response or specific mechanisms ?” Silvestre F. Beltox Annual Conference, 25th of November 2010, Louvain-la-Neuve, Belgium. 3° Other oral communications “Environmental proteomics to predict the effects of global warming on aquatic organisms exposed to pollutants” Silvestre F. and Dorts J. 6th SETAC World Congress, Berlin (Germany), 20th-24th May 2012. “Mechanisms of action of toxicants on aquatic animals. Does expression proteomics reveal general stress response or specific mechanisms ?” Silvestre F. PRIMO 16th meeting, Long Beach (California), 15th-18th May 2011.

“Effects of Roundup® on the protein expression patterns in the liver and brain of metamorphosing common frog Rana temporaria.” Gillardin Virginie, Silvestre Frédéric, Oriol Annick, Dieu Marc, Raes Martine and Kestemont Patrick. SETAC Europe 21st Annual Meeting, Milan (Italy), 15-19 May 2011. “Molecular responses of lymphocytes in European eel Anguilla anguilla exposed to PFOS. Biomarker discovery using a sub-proteomic approach.” Roland K., Kestemont P., Pierrard M.-A., DieuM., Raes M., Silvestre F. SETAC Europe 21st Annual Meeting, Milan (Italy), 15-19 May 2011. “Searching for reliable biomarkers of malachite green exposure in Pangasianodon hypophthalmus by a non invasive approach.” Pierrard M.-A., Kestemont P., Delaive E., Dieu M., Phuang T.H., Raes M., Silvestre F. SETAC Europe 21st Annual Meeting, Milan (Italy), 15-19 May 2011. “Combined effects of global warming and cadmium exposure in bullhead Cottus gobio”. Jennifer Dorts, Patrick Kestemont, Marc Dieu, Martine Raes, Frédéric Silvestre. SETAC Europe 20th Annual Meeting, Seville (Spain), 23-27 May 2010. “Effects of the PolyChlorinatedBiphenyls mixture Aroclor 1254 on the development of Xenopus laevis: what did we learned from an ecotoxicological approach using proteomics”. Virginie Gillardin, Frédéric Silvestre, Kathleen Roland, Jean-Pierre Thomé, Martine Raes, and Patrick Kestemont. SETAC Europe 20th Annual Meeting, Seville (Spain), 23-27 May 2010. “A protein expression signature as multi-biomarker on leucocytes from Asian catfish and goldfish to highlight exposure to malachite green”. M-A. Pierrard, E. Delaive, P. Kestemont, F. Silvestre. Beltox annual meeting, Edegem (Belgium), 25 November 2009. “Acute and chronic exposure to Aroclor 1254 impair protein expression profiles in Xenopus laevis tadpoles during early development and metamorphosis”. Gillardin, V., Silvestre, F., Kestemont, P. SETAC Europe 17th Annual Meeting, Porto (Portugal), 20-24 May 2007.

“Oxidative stress and protein carbonylation in Penaeus monodon exposed to pesticides in Vietnam”. Dorts J., Silvestre, F., Tu, H.T., Kestemont, P. 13th Benelux Congress of Zoology, Leuven, 27-28 October 2006.

“ Mechanisms of acclimation to cadmium in the Chinese mitten crab Eriocheir sinensis. An integrative approach including analysis of the proteome ». Silvestre F., Dierick, J.-F., Trausch, G., Raes, M., Devos, P., Kestemont, P. SETAC Europe 16th Annual Meeting, La Haye (The Netherlands), 7-11 May 2006. « Proteomic analysis of the cadmium response in anterior gills of the Chinese mitten crab, Eriocheir sinensis”. F. Silvestre, V. Dumont, M. Dieu, M. Raes, P. Devos. 23rd ESCPB conference: Coping with environmental factors at sea: a molecular approach. Cesenatico (Italy), 23-26 September 2004. « Proteomics analysis to characterize the stress response of the Chinese mitten crab, Eriocheir sinensis, after cadmium contamination “. F. Silvestre. Workshop Beltox: Role of bioavailability in environmental risk assessment. Liège (Belgium), 15 May 2004. « Proteomics analysis to characterize the stress response of the Chinese mitten crab, Eriocheir sinensis, after cadmium contamination ». F. Silvestre, J.-F. Dierick, E. Delaive, M. Albert, P. Devos. 22nd ESCPB conference : Biological effects of pollutants, the role of environmental proteomics and genomics. Alessandria (Italy), 14-18 December 2003. « Proteomics analysis in ecotoxicology : an emerging technique to study the responses of aquatic organisms submitted to environmental stressors ». F. Silvestre. Workshop sur « Recent progress in endocrine disruption and toxicity testing », KUL, Leuven (Belgium), 16-17 September 2002. « Effects of cadmium on blood osmoregulation and gill ultrastructure of the chinese crab, Eriocheir sinensis ». F. Silvestre, P. Compère, A. Péqueux.

2nd european conference on crustaceans, Liège (Belgium), 2-6 September 1996. 2° Posters of the last 3 years “Looking for stable biomarkers in the PBMC of Asian catfish, Pangasianodon hypophthalmus, following malachite green exposure using a proteomic approach.” Marie-Aline Pierrard, Patrick Kestemont, Edouard Delaive, Marc Dieu, Nguyen Thanh Phuong, Martine Raes and Frédéric Silvestre. Beltox Annual Conference, 25th of November 2010, Louvain-la-Neuve, Belgium. “Wap65 is reduced during cadmium acclimation in least killifish, Heterandria formosa, as revealed by sub-proteomic analysis”. Frédéric Silvestre, Joseph Adeyemi, Marc Dieu, Martine Raes, Patrick Kestemont, Paul Klerks. SETAC Europe 20th Annual Meeting, Seville (Spain), 23-27 May 2010. “Molecular responses of lymphocytes in European eel exposed to cadmium. Biomarker discovery using combined expression and redox sub-proteomics approaches”. Kathleen Roland, Patrick Kestemont, Frédéric Silvestre. SETAC Europe 20th Annual Meeting, Seville (Spain), 23-27 May 2010. “Molecular responses of lymphocytes in European eel Anguilla anguilla exposed to different xenobiotics. Development of a non invasive multi-biomarkers approach using a sub-proteomic analysis.” K. Roland, P. Kestemont, M.-A. Pierrard, F. Silvestre. Beltox annual meeting, Edegem (Belgium), 25 November 2009. “Development of a molecular biomarker system using proteomics on lymphocyte culture to assess the use of drugs and chemicals in Pangasianodon hypophthalmus aquaculture”. M-A. Pierrard, P. Kestemont, F. Silvestre. !"#$%&'()*+,-%(.+/0,112%3++1405672%851#09:2%";<%',=#,>4,02%!??<@% “'54;A,#$:A%6:B>95>%#.C969#D%#.%45AA$,:B%!"##$%&'"()"@%*9.6$,>96:A%:+B%=0.#,.>96%:==0.:6$,1E@%F@%G.0#12%)@%H,1#,>.+#2%I@%G9,52%I@%J:,12%K@%'9AL,1#0,@%!"#$%&'()*+,-%(.+/0,112%3++1405672%851#09:2%";<%',=#,>4,02%!??<@% “Dissimilar short-term and long-term acclimation mechanisms to cadmium in least killifish, Heterandria formosa, revealed by sub-proteomic analysis”. Silvestre, F., Adeyemi, J., Gillardin, V., Kestemont, P., Klerks, P. SETAC North America 29th Annual Meeting, Tampa, FL (USA), 16-20 November 2008. “Protein expression profiles as biomarker of environmental stress in two endemic sturgeon species of San Francisco Bay Delta”. Madison, R., Silvestre, F., Kueltz, D. SETAC North America 29th Annual Meeting, Tampa, FL (USA), 16-20 November 2008. %M(.>49+,B% ,NN,6#1% .N% /A.4:A% -:0>9+/% :66A9>:#9.+% :+B% C,+.49.#96% ,C=.150,% 9+% 45AA$,:B%!"##$%& '"()"*@% F@%G.0#12%K@%'9AL,1#0,2%)@%H,1#,>.+#@%%BELTOX annual meeting, Woluwé (Belgium), 28 November 2008. “Chronic exposure to aroclor 1254 impairs expression profiles in the developing brain of xenopus laevis tadpoles”. Gillardin, V., Silvestre, F., Roland, K., Raes, M., Kestemont, P. BELTOX annual meeting, Woluwé (Belgium), 28 November 2008. “ Chronic exposure to aroclor 1254 impairs expression profiles in the developing brain of Xenopus laevis tadpoles”. Gillardin, V., Silvestre, F., Roland, K., Raes, M., Kestemont, P. SETAC Europe 18th Annual Meeting, Warsaw (Poland), 25-29 May 2008. “Combined effect of global warming acclimation and xenobiotic exposure in bullhead Cottus gobio”. Dorts, J., Silvestre, F., Kestemont, P. Beltox-BLT annual meeting, Brussels, 28 November 2008.

Expertises

! Member of the jury committee for 9 PhD thesis :

o Marleen Eyckmans “Defense mechanisms of fish with different sensitivities to copper: in depth analysis of gill physiology.” University of Antwerp (Belgium) – May 2012

o Marie-Aline Pierrard “Responses of peripheral blood mononuclear cells to malachite green in the Asian catfish combining in vitro and in vivo approaches.” University of Namur FUNDP (Belgium) – December 2011

o Joseph Adeyemi “Physiological and biochemical aspects of copper toxicity in copper or salinity acclimated fish.” University of Louisiana at Lafayette, USA. November 2011.

o Marie-Dorothée Castets “Fonction de reproduction et régulation de la qualité chez la perche commune Perca fluviatilis.” Institut National Polytechnique de Lorraine, France. November 2011.

o Jennifer Dorts “Combined effects of heat stress and xenobiotic exposure in the European bullhead Cottus gobio. Clues provided by enzymatic and proteomic approaches.” University of Namur FUNDP (Belgium) – October 2011

o Joseph Schnitzler “Approaches for assessing the presence and impact of thyroid hormone disrupting chemicals in sea bass from European coasts”. University of Liège (Belgium) – January 2011.

o Virginie Gillardin “Effects of PCBs on developing amphibian tadpoles : an integrative approach using proteomic analysis”. FUNDP – November 2009.

o Tu Huynh Thi “The use of biomarkers in black tiger shrimp in Vietnam”. University of Namur FUNDP (Belgium) – March 2009.

o Neïla Hamza “Influence des facteurs alimentaires et nutritionnels sur la mise en place des structures digestives et l’expression protéique durant l’ontogenèse larvaire du sandre Sander lucioperca (Percidae; Linné, 1758). University of Namur FUNDP (Belgium) – March 2008.

! Reviewer for the following scientific journals :

! Applied Genomics and Proteomics ! Aquatic Toxicology ! Chemosphere ! Comparative Biochemistry and Physiology ! Ecotoxicology ! Environmental Research ! Environmental Toxicology ! Environmental Toxicology and Chemistry ! Fish Physiology and Biochemistry ! Journal of Experimental Marine Biology and Ecology ! Journal of Proteome Research ! Molecular and Cellular Proteomics ! Proteomics

! Member of the jury committee for the FRIA grants, Belgium. Commission « Zoology ». Since 2011.

! Member of the jury committee for the “Adrien Bauchau Award” of the best graduate

thesis in biology (FUNDP-2005 and 2010-?).

! Expert for research projects submitted to the Brittany Region (France) – Regional policy for the strengthening of research.

Societies

! ISEO (International Society of Environmental Omics) ! SETAC (Society for Environmental Toxicology and Chemistry) ! FNRS contact group in proteomics ! Fulbright Scholar Program Alumni

Accepted research projects

”Aquatic systems under multiple stress: a new paradigm integrating aquaculture and ecotoxicology research. ” Project IAP (Interuniversity attraction poles – Belspo). 2012-2017. Partner. ” Transgenerational impacts of xenobiotics in aquatic vertebrates. Epigenetic, proteomic, endocrine and structural physico-chemical combined approaches.” Project FRFC (Fonds de la Recherche Scientifique). 2011-2015. Leading promotor. ” Development of a multi-biomarker assay using a proteomic approach in Danio rerio to assess the ecotoxicity of chemicals under REACH legislation”. First Spin Off grant from the Walloon Regional Government for the setup of a Spin Off company. Co-Promotor, 2009-2012. ” Integrated Study of the impacts of pollutants on the nervous system of the European eel, Anguilla anguilla”. Project FRFC (Fonds de la Recherche Scientifique). 2010-2014. Partner. “Acclimation and adaptation mechanisms to cadmium in aquatic organisms. Sub-proteomic analysis of the endoplasmic reticulum and heritability of observed phenotypes. Implication of epigenetic mechanisms ». FNRS post-doctoral grant. 1/2007-10/2010. “Effects of an herbicide (Roundup) on behaviour, physiology and development of amphibians”. Project FRFC (Fonds de la Recherche Scientifique). 2008-2012. Partner. “Analytical and biological methods in support of sustainable aquaculture practices in Vietnam”. Scientific cooperation between the Vietnamese Ministry of Science and Technology (MOST) and the Federal Science Policy Office (BSPO). 2007-2009. “Improvement of shrimp production sustainability and shrimp safety in Vietnam. Workpackage 3 : Study of the effects of chemicals and drugs on the physiology and health of cultivated and wild shrimps”. Scientific cooperation between the Vietnamese Ministry of Science and Technology (MOST) and the Belgian Federal Science Policy Office (BELSPO). From april 2004 to april 2006.

Main scientific collaborations

! Research Unit in Cell Biology (Pr. M. Raes – University of Namur, Belgium) ! Unit of theoritical and structural physico-chemistry (Dr. E. Perpète et C. Michaux –

University of Namur, Belgium) ! Department of Biology (Pr. Ronny Blust – University of Antwerp, Belgium) ! Animal Biology Unit (Pr. J.-F. Rees – Catholic University of Louvain, Belgium) ! Laboratory of Oceanology (Dr. K. Das – University of Liège, Belgium) ! Analysis Center of Trace Residues (Pr. M-T. Scippo and J.-P. Thomé – University of

Liège, Belgium) ! Ethology and animal psychology (Dr. Mathieu Denoël - University of Liège, Belgium) ! Institut National de l’Environnement et des Risques (Dr. Wilfried Sanchez - INERIS,

France) ! UMR 5119 Ecosym (Pr. G. Charmantier – University of Montpellier 2, France) ! College of Aquacultue and Fisheries (Pr. N. T. Phuong – University of CanTho –

Vietnam) ! Department of Biology (Pr. P. Klerks – University of Louisiana Lafayette – USA) ! Department of Animal Sciences and Genome Center (Pr. D. Kültz – University of

California Davis – USA) ! Department of Biological Sciences (Pr. R.L. Earley – University of Alabama – USA) ! Environmental Proteomics Laboratory (Pr. L. Tomanek – California Polytechnic State

University – USA) ! Department of Biology (Pr. W. Dowd – Loyola Marymount University – CA – USA)

Main techniques

! SDS-PAGE in 1 and 2 dimensions (EttanDalt and Criterion systems) ; NuPAGE ! DIGE analysis (Differential in Gel Electrophoresis) - Typhoon 9400 Imager - EttanTM Spot

Picker ! Protein carbonylation detection using 2D gels ! Epigenetic approaches (DNA methylation, histone proteomics) – in progress ! Mass spectrometry (ABI 4700 Maldi TOF TOF ; LC-MS/MS Q-TOF) ! Bioinformatic analysis of proteomic results : Decyder, Image Master, Delta 2D,

PermutMatrix, SAM, Scaffold 2, ProteinScape 2, Mascot 2.0., MS Blast, Blast2GO, GPL, Panther, Pfam, Uniprot.

! Western-blots and immunoblotting techniques ! Enzymatic activities assessment (oxidative stress enzymes ; EROD/ECOD ; AChE ;

Na+/K+-ATPase ; cytochrome c oxidase ; …) ! Subcellular fractionation (differential centrifugation) ! Metallothioneins silver-saturation assay ! Heavy metals analysis by atomic absorption and ICP mass spectrometry ! Gills perfusion and radioisotopes fluxes

PhD student supervision

“ Delayed effects of pollutants on aquatic organisms.” Ph.D thesis of Elodie Falisse, FUNDP (teaching Assistant sonce 2011). Promotor “ Molecular responses of lymphocytes in European eel Anguilla anguilla exposed to different xenobiotics. Development of a non invasive multi-biomarkers approach by a sub-proteomic analysis “. Ph.D thesis of Kathleen Roland, FUNDP (FRIA since 2008). Co-promotor “ Cellular response of lymphocytes from Pangasius hypophthalmus following the use of xenobiotics in aquaculture. Development of a non-invasive multi-biomarker approach using sub-proteomics “. Ph.D thesis of Marie-Aline Pierrard, FUNDP (FRIA since 2007). Co-promotor “ Combined effects of an acclimation to global warming and an exposure to a xenobiotic in Cottus gobio. Mitochondrial sub-proteomic analysis and epigenetic regulations. ". Ph.D thesis of Jennifer Dorts, FUNDP (aspirante FNRS since 2007). Co-promotor “ Acclimation mechanisms to metals in fish “. Ph.D thesis of Joseph Adeyemi, University of Louisiana at Lafayette (teaching assistant since 2007). “ Effects of PCBs on developing amphibian tadpoles : an integrative approach using proteomic analysis “. Ph.D thesis of Virginie Gillardin, FUNDP (graduated in January 2010). “ The use of biomarkers in shrimp aquaculture in Vietnam “. Ph.D thesis of Huynh Thi Tu, FUNDP (graduated in March 2009).

Teaching activities

1° Lectures

Ecotoxicology - MS in Organism Biology and Ecology – University of Namur FUNDP – 12h (since 2011)

Animal Physiology - BS Biology - University of Namur FUNDP – 10h (since 2011) Comparative Animal Physiology – BS Biology - University of Namur FUNDP – 14h

(since 2012) Evolution - MS in Molecular and Cellular Biology - University of Namur FUNDP – 7.5h

(since 2011) Animal Biology – BS Pharmacy, Veterinary and Biomedicals. University of Namur

FUNDP – 35h (since 2010) Ecophysiology – BS Biology - University of Namur FUNDP – 15h (since 2010) Genomics and Proteomics – MS in Organism Biology and Ecology – University of

Namur FUNDP – 6h (since 2007) Relations animals/environment - MS in Organism Biology and Ecology – University of

Namur FUND – 18h (since 2007) Theoretical and practical training at the University of CanTho (Vietnam) for professionals

– «The use of biomarkers in environmental sciences and aquaculture » (3 days in 2006)

2° Practical training

Zoology – BS biology - ± 155h/year (from 1998 to 2004)

Zoology – BS geology – ± 15h/year (from 1998 to 2004)

Human Anatomy and Physiology – BS pharmacology – ± 30h (1998)

Ecophysiology – BS biology - ± 10h/year (from 2000 to 2004)

Ecotoxicology – MS biology - ± 10h/year (from 2000 to 2004)

Field training in Marine Biology – BS biology - 6 days/year (Brittany-F) (from 1998 to 2005)

3° Master degree thesis supervision

Anne-Sophie Voisin (2012) Leonor Almeida (2012) Antoine Dubois (2011) Aurélie Bauwin (2011) Noémie Nkejabega (2007) Bertrand De Meulder (2007) Jennifer Dorts (2006) Anne-Erick Tyberghein (2006) Laure Berlage (2006) Amandine Bernard (2005) Caroline Duchêne (2004)

Scientific vulgarisation activities Lecture : “ Can functional genomic and epigenetic rehabilitate some evolutionary theories of Lamarck ?” Namur, January 2008 (“Which life on an impoverished planet ?” www.whichlife.be ) Coordination of a round-table about “international tourism and animal kingdom”, 1st exhibition on alternative and ethic tourism, Brussels, 21-22 October 2006. Coordination of an exhibition about Biodiversity, during « Bio 2001 », FUNDP Namur, January 2001. http://www.fundp.ac.be/bio2001/bioscope/biodiversite/biodiversite.html Lecture : «Ecotourism in Antarctica : a tool for sustainable development? » , Brussels, October 1998. Article in « Le Vif/L’Express » of November 6th 1998 about ecotourism in Antarctica.

Services and other skills o Member of the Faculty of Sciences Board, University of Namur FUNDP, Belgium (2003-

2005 and since 2010). o Coordinator of the research group on sustainable development – Biodiversity and natural

resources conservation, University of Namur FUNDP, Belgium (since 2010). o Academic coordinator for internship of MS students in Organism Biology and Ecology -

University of Namur FUNDP, Belgium (since 2010). o Setup and management of an environmental proteomics core in the Research Unit in

Environmental and Evolutionary Biology (URBE), University of Namur FUNDP, Belgium.

o Co-promotor of an applied research project aiming at developing a spin off company in the field of Ecotoxicology under REACH legislation

o Management of a project related to improving the sustainability of Vietnamese aquaculture - impacts of antibiotics and pesticides - development of biomarkers - 9/2004-8/2006

o Setup of a new field class in marine biology for undergraduate students. Six days in Brittany, France.

o Entrepreneurship and leadership o Research project management and research team management o Creativity and good capacity of adaptation o Driving licence o Fluent french/english; some dutch Other interests o Ecotourism : South West USA; California; Florida; Mexico; Cuba; Spain; Red Sea;

Australia, Vietnam, Namibia… o Photography: http://www.fsilvestrephoto.be o Sports: outdoor sports ; SCUBA diving (1 star CMAS)